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The 2015 version of the "Cosmetics Safety Technical Specifications" requires that the total number of bacteria and the total number of molds and yeasts are mainly tested for microorganisms.

On the premise that the total number is controlled below the standard, no heat-resistant coliform bacteria, Pseudomonas aeruginosa, and golden yellow staphylococcus. After cosmetics are contaminated by pathogenic bacteria, it will not only affect the good brand image of the product, but also cause a fatal blow to the company's economy, but also cause serious harm to the customer's body. Therefore, no matter which country it is, they are very concerned about the quality and safety of cosmetics. Every country or region in the world has implemented its own country's cosmetic microbial content standards, and clearly stipulated the content of microorganisms, so as to maximize the Reducing the pollution of cosmetics caused by viruses produced by microorganisms can not only improve their quality and safety, but also protect the health of consumers



Detection principle


The quality of any cosmetic or drug may change due to various reasons during production, storage, transportation and use. One of these reasons is caused by the so-called microorganisms, which are a group of extremely small organisms, which generally include bacteria, yeasts, molds and viruses. Cosmetics or drugs are contaminated by microorganisms, which can cause changes in the physical properties of the products (such as color, odor, etc.), change the active ingredients of the products, and sometimes cause allergies, toxins and virus infections. Therefore, microorganisms in cosmetics or drugs must be strictly controlled. .


The total number of colonies refers to the total number of colonies contained in 1g (1mL) of the test sample after being processed and cultured under certain conditions (such as medium composition, culture temperature, culture time, pH value, aerobic properties, etc.). The results obtained include only the total number of mesophilic aerobic colonies grown under the conditions specified in this method.


The total number of molds and yeasts refers to the total number of live molds and yeast colonies in 1g or 1mL of cosmetics after the sample is cultured under certain conditions, so as to determine the degree of mold and yeast contamination of cosmetics and their general hygiene. According to the unique morphology and culture characteristics of molds and yeasts, the method was cultured at 28°C for 72 hours on tiger red medium, and the number of molds and yeasts grown was calculated.









Cosmetics and Microorganisms


Every time a function is added to cosmetics, its raw material components will also increase, and the raw material environment will become a high-quality development cradle for the reproduction of these pathogenic bacteria, because these raw materials have various nutrients such as carbon sources. And cosmetics are basically composed of water, and a humid environment is conducive to the growth and reproduction of pathogenic bacteria. In addition, the pH of cosmetics is between PH4-7, and the storage temperature is 20~30℃, which are close to the growth conditions of pathogenic bacteria such as bacteria and mold. The above factors all provide conditions for the survival and reproduction of pathogenic bacteria in cosmetics. In inferior cosmetics, the pathogenic bacteria exceeding the standard or the color change, smell and mildew during the storage process will cause the user to be sick and infected to varying degrees. Therefore, the detection and control of cosmetic products Microorganisms are extremely important


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